effects of mntnhex-2-pyp on lung antioxidant defence system in asthma mice model.
نویسندگان
چکیده
we aimed to study the mntnhex-2-pyp effect on some markers of lung antioxidant defence system in mice asthma model.the study was carried out on 28 c57b1/6 mice divided into four treatment groups: group 1 - controls; group 2 - injected and inhaled with ovalbumin; group 3 - treated with mntnhex-2-pyp and inhaled with phosphate buffered saline; group 4 - injected with ovalbumin and mntnhex-2-pyp but also inhaled with ovalbumin. on days 24, 25 and 26, mice from groups 1 and 2 were inhaled with pbs for 30 min, and those from groups 2 and 4 were given a 1% ovalbumin solution. one hour before inhalation, and 12 hours later the animals from groups 1 and 2 were injected i.p. with 100 μl pbs, and those from groups 3 and 4 received a 100 μl mntnhex-2-pyp solution in pbs, сontaining 0,05mg/kg. the animals were killed by exsanguination 48 hours after the last inhalation for obtaining a lung homogenate. the activities of superoxide dismutase, catalase, glutathione peroxidase and the non-protein sulphhydryl group content in the lung homogenate were investigated. ovalbumin decreased the activities of superoxide dismutase (p=0.01), catalase (p=0.002), glutathione peroxidase and non-protein sulphhydryl groups content (p<0.001) in comparison to controls. in group 4 (ovalbumin and mntnhex-2-pyp) the activities of superoxide dismutase (p=0.044), catalase (p=0.045), glutathione peroxidase (p=0.002), and the non-protein sulphhydryl groups content (p<0.001) were significantly increased compared to ovalbumin (group 2).mntnhex-2-pyp restored the activities of basic enzymes in the lung antioxidant defence system in ovalbumin-induced asthma mice model, 48 hours after the last nebulization.
منابع مشابه
Effects of MnTnHex-2-PyP on lung antioxidant defence system in asthma mice model.
We aimed to study the MnTnHex-2-PyP effect on some markers of lung antioxidant defence system in mice asthma model.The study was carried out on 28 C57B1/6 mice divided into four treatment groups: group 1 - controls; group 2 - injected and inhaled with ovalbumin; group 3 - treated with MnTnHex-2-PyP and inhaled with phosphate buffered saline; group 4 - injected with ovalbumin and MnTnHex-2-PyP b...
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عنوان ژورنال:
iranian journal of allergy, asthma and immunologyجلد ۱۱، شماره ۴، صفحات ۳۲۹-۳۳۵
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